By David Puett, Krassimira Angelova (auth.), Ok-Kyong Park-Sarge, Thomas E. Curry (eds.)
Over the process the final hundred years, the sphere of endocrinology has got ever-increasing recognition. learn and constructing applied sciences have led to an important bounce in our realizing of the motives and therapy of endocrine disorder. In Molecular Endocrinology: tools and Protocols, leaders within the box proportion a range of state of the art ideas which are turning into frequently hired within the quest to additional comprehend hormone motion. with the intention to achieve the widest variety of labs, this quantity contains protocols that let investigators in any respect phases in their clinical profession to effectively practice those recommendations. Written within the hugely profitable Methods in Molecular Biology™ sequence layout, chapters contain short introductions to their respective topics, lists of the required fabrics and reagents, step by step, simply reproducible laboratory protocols, and professional notes on troubleshooting and warding off identified pitfalls.
Comprehensive and simple to exploit, Molecular Endocrinology: tools and Protocols is a perfect consultant for researchers in labs internationally who search a deeper knowing of the advanced strategies and biology of hormones.
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Extra resources for Molecular Endocrinology: Methods and Protocols
For example, the specific counts bound ¼ total counts À non-specific counts. From a knowledge of the specific radioactivity of the iodinated hormone, the counter efficiency, and experimental conditions, the concentration in nM or pM can be determined. 7. The kinetics of hormone–receptor internalization should be determined to ensure that the observed specific binding is attributable to cell surface receptor binding and has little or no contribution from internalized complexes (see ref. 15 for methodologies).
16. mechanisms, and different types and mechanisms of inhibition. Mol. Pharmacol. 10, 235–247. , and Puett, D. (2008) Luteinizing hormone-induced up-regulation of ErbB-2 is insufficient stimulant of growth and invasion in ovarian cancer cells. Mol. Cancer Res. 6, 1775–1785. Galet, C. and Ascoli, M. (2005) The differential binding affinities of the luteinizing hormone (LH)/choriogonadotropin receptor for LH and choriogonadotropin are dictated by different extracellular domain residues. Mol. Endocrinol.
The linearity of the assay varies from 91 to 113% (see package insert for dilutions). 15. Distilled water of consistent quality is required. Water should meet NCCLS Type 1 reagent water standards at the time of preparation. 16. An alarm will sound when the bottle of chemiluminescent substrate is low. There will be enough chemiluminescent substrate in the Immulite to run 20–25 test units once the alarm sounds. The Immulite WILL run with the chemiluminescent substrate bottle removed. 17. While the minimum sample volume required for estradiol assay by the Immulite 1000 is 125 ml, we recommended using at least 150 ml.