Download Molecular Endocrinology: Methods and Protocols by David Puett, Krassimira Angelova (auth.), Ok-Kyong PDF

By David Puett, Krassimira Angelova (auth.), Ok-Kyong Park-Sarge, Thomas E. Curry (eds.)

Over the process the final hundred years, the sphere of endocrinology has got ever-increasing recognition. learn and constructing applied sciences have led to an important bounce in our realizing of the motives and therapy of endocrine disorder. In Molecular Endocrinology: tools and Protocols, leaders within the box proportion a range of state of the art ideas which are turning into frequently hired within the quest to additional comprehend hormone motion. with the intention to achieve the widest variety of labs, this quantity contains protocols that let investigators in any respect phases in their clinical profession to effectively practice those recommendations. Written within the hugely profitable Methods in Molecular Biology™ sequence layout, chapters contain short introductions to their respective topics, lists of the required fabrics and reagents, step by step, simply reproducible laboratory protocols, and professional notes on troubleshooting and warding off identified pitfalls.

Comprehensive and simple to exploit, Molecular Endocrinology: tools and Protocols is a perfect consultant for researchers in labs internationally who search a deeper knowing of the advanced strategies and biology of hormones.

Show description

Read or Download Molecular Endocrinology: Methods and Protocols PDF

Best endocrinology books

The Foot in Diabetes, 3rd Edition

Книга The Foot in Diabetes, third variation The Foot in Diabetes, third EditionКниги Медицина Автор: A. J. M. Boulton, Henry Connor, P. R. Cavanagh Год издания: 2000 Формат: pdf Издат. :John Wiley & Sons Страниц: 388 Размер: 4,3 ISBN: 0471489743 Язык: Английский0 (голосов: zero) Оценка:Reviews from prior versions: "A publication that are meant to discover a position at the bookshelves of all physicians who take care of diabetic sufferers.

Ultrasound Diagnostics of Thyroid Diseases

This booklet relies at the authors’ broad useful adventure within the use of recent ultrasound, and different radiological equipment, within the analysis of thyroid ailments. The authors have analyzed greater than 100,000 ultrasound examinations played among 1995 and 2008 in sufferers with thyroid and parathyroid illness, in addition to many millions of diagnostic and healing ultrasound-guided minimally invasive systems.

Nuclear endocrinology

Nuclear drugs is a vital section of day-by-day perform for the endocrinologist, either for analysis and for remedy. the continual swift improvement of nuclear drugs systems has created the necessity for a concise, up to date functional consultant that provides the basic details required through the endocrinologist.

Calcium: The Molecular Basis of Calcium Action in Biology and Medicine

The large and sundry function of calcium in dwelling structures is now extensively preferred by way of either cellphone biologists and clinicians. The id and characterisation of recent calcium binding proteins and regulatory pathways is matched via the popularity of the involvement of calcium binding proteins in more and more sickness states.

Extra resources for Molecular Endocrinology: Methods and Protocols

Example text

For example, the specific counts bound ¼ total counts À non-specific counts. From a knowledge of the specific radioactivity of the iodinated hormone, the counter efficiency, and experimental conditions, the concentration in nM or pM can be determined. 7. The kinetics of hormone–receptor internalization should be determined to ensure that the observed specific binding is attributable to cell surface receptor binding and has little or no contribution from internalized complexes (see ref. 15 for methodologies).

16. mechanisms, and different types and mechanisms of inhibition. Mol. Pharmacol. 10, 235–247. , and Puett, D. (2008) Luteinizing hormone-induced up-regulation of ErbB-2 is insufficient stimulant of growth and invasion in ovarian cancer cells. Mol. Cancer Res. 6, 1775–1785. Galet, C. and Ascoli, M. (2005) The differential binding affinities of the luteinizing hormone (LH)/choriogonadotropin receptor for LH and choriogonadotropin are dictated by different extracellular domain residues. Mol. Endocrinol.

The linearity of the assay varies from 91 to 113% (see package insert for dilutions). 15. Distilled water of consistent quality is required. Water should meet NCCLS Type 1 reagent water standards at the time of preparation. 16. An alarm will sound when the bottle of chemiluminescent substrate is low. There will be enough chemiluminescent substrate in the Immulite to run 20–25 test units once the alarm sounds. The Immulite WILL run with the chemiluminescent substrate bottle removed. 17. While the minimum sample volume required for estradiol assay by the Immulite 1000 is 125 ml, we recommended using at least 150 ml.

Download PDF sample

Rated 4.32 of 5 – based on 41 votes